It's not possible to tell based on that picture whether the conflict should've been displayed or not. As at least DLH22AK is flagged non-RVSM, the higher vertical separation applies. As apparently in your setup you've set the separation value to exactly 2000ft, it's enough for the predicted separation to be anything less than that for the conflict to be displayed (the default upper/lower separation values are 1800/800ft to account for small variations in the level predictions).
If you saved a logfile, you can go back to that situation and see what might have caused the alert. The default EuroScope tags' route prediction can be toggled to display the predicted altitudes along the route. If the predictions show even one foot less separation than 2000ft, then increasing the separation values should help (and I'd recommend it in any case). If not, then it's something else. Then make a dump file from that moment and send it to me. If there's no log file, there's no way to debug the issue.